Ap Biology Lab free essay sample

Explain how environmental factors affect the rate of enzyme-catalyzed reaction. Explain why catalysis activity decreased over time. 3. Introduction †¢ An enzyme is a specific organic catalyst (substance that speeds up reaction without being used up in the experiment) in which it binds on to a specific substrate (reactant) at the active site and lowers the activation energy required to perform that reaction. Enzymes are important biologically because, for example, the human body performs certain reactions that keep us alive and most of them react at 37 degrees Celsius (body temperature. In each of these reactions, there is an enzyme that catalyzes these reactions to lower the amount of energy needed to perform this reaction. If enzymes didn’t exist then body temperature wouldn’t be enough to perform any of these reactions properly. Enzymes have certain conditions in which it will work in. For instance, most enzymes react at certain temperatures (varies in each enzyme), most enzymes react from a pH of 6- 8, and react faster or slower according to the amount of substrate present. We will write a custom essay sample on Ap Biology Lab or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Altering temperature, pH level, and concentrations of enzyme and substrates may cause absolute no change to the activation energy in which the enzyme would have been denatured. In addition to these environmental factors, other molecules may react with an enzyme. These may have great to no effect on the enzymes. Those that increase the rate of substrate entrance into the active site are known as activators by hitting the enzyme on one side of the protein (enzymes are protein) and altering the shape of the active site on the other side. Those molecules that slow down or stop activation are known as inhibitors which bind on to one side of the protein and cause the active site to misshapen which would decrease or eliminate the amount of substrates that may enter the active site. 4. Materials and Methods †¢ See lab notebook pages. 5. Results 2a: 1. Observe the reaction a. What is the enzyme in this reaction? †¢ Catalase solution b. What is the substrate in this reaction? †¢ H2O2 c. What is the product in this reaction? †¢ H20 and 02 d. How could you show that the gas evolved is 02 Capture bubbles in closed solution and ignite. If flame appears then if 02 2. Explain the reason for this difference. †¢ First reaction had 02 gas bubbles and the second one did not have bubbles. 3. What do you observe? What do you think would happen if the liver was boiled before being added to the H2O2? †¢ Gaseous bubbles formed around the liver. If liver had been boiled there would be no bubbles. 2b: Baseline calculation Final reading of burette 22. 5 mL Initial reading of burette 26. 5 mL Baseline (initial- final) 4 mL 2c: Uncatalyzed H2O2 decomposition Explain your prediction. a. Lowering the temperature slow the rate of enzyme activity because of the effect on the activation energy. 6. Design a controlled experiment to test the effect of varying pH, temperature, or enzyme concentration. a. A controlled experiment that would test pH could include comparing two groups, a control group with no added acid, and an experimental group with added acid. Each group would consist of a solution containing an enzyme such as catalase and the material it breaks down such as H2O2. The independent variable would be the added acid while the dependent variable would be the difference in amount of H2O2 broken down. This could be measured through a titration of KMnO4. Discussion: Experimental results in 2D did not support conclusion effectively. Conclusion should’ve reflected that when time is a factor, more and more substrate gets used up by the enzyme. The reason is because when the titration was taking place, different people were doing it which would affect the credibility and precision of the titration. This false procedure caused the results to vary and fluctuate accordingly to the mistake taking place.

Free Essays on Hazing

Hazing In today’s world if a person would want to get on a certain team or join a frat or sorority, that person would have to put up with one big thing, initiation. Or as most call it hazing. There not the only people that put up with hazing though. Hazing is becoming big in high schools, church events, and even professional sports. Since so many people have to put up with the issue of hazing, it has become a very big issue in today’s world. Whether it involves binge drinking, kidnapping, or some type of physical abuse, hazing is becoming an issue that is too common. Since it is becoming so common most people feel that someone needs to step in and stop hazing in all events no matter what it is. Hazing can be defined in many different ways depending on whom you’re talking to. But the actual definition is that it is any activity or situation that has been formed with the intention of causing another person mental or physical pain, embarrassment, or ridicule. Hazing can range from making a person eat ridiculous things, to even have a person take a beating from others. Hazing happens mostly in colleges, but is becoming more common with high schools and other organizations as well. People that want to join a frat or sorority have to deal with this issue on a daily basis, as well as athletes. The idea of hazing is to show how much a certain individual wants to be in a certain club. So since the people at the head of the club can pretty much do what ever they want to a certain individual, they do. They do that because it deals with the universal saying, only the strong survive. Which means the only people that will make it into the club are the ones that do whatever they say. So the heads of the clubs have each person wanting to join do outrageous things because they want to show there power as well as make the person look stupid. But that’s why the heads of these clubs make the statement that it is supposed to g... Free Essays on Hazing Free Essays on Hazing When one hears the word â€Å"hazing,† the typically first impression is that of a fraternity member with a paddle in one hand, beer in another and a young â€Å"pledge† bent over being struck by the wooden paddle. This is defiantly a case of classic hazing, however many other forms have been created and made public in the recent past. Other fields such as military, sorority, high school and athletic teams have begun to come under the same scrutiny fraternities have been for years. For someone who has never been â€Å"hazed† the issue can be quite complicated and some ignorant conclusions may be made. Hazing has different, but yet very similar definitions from various institutions. The first thing I noticed here was that fraternities were the only institution that the American College Dictionary directly addresses. Obviously the FIPG listed above goes into more detail regarding fraternity actions and focus on those activities Hazing is defined by the FIPG (Fraternity Insurance Purchasing Group) as: "Any action taken or situation created, intentionally, whether on or off fraternity premises, to produce mental or physical discomfort, embarrassment, harassment, or ridicule. Such activities may include but are not limited to the following: use of alcohol; paddling in any form; creation of excessive fatigue; physical and psychological shocks; quests, treasure hunts, scavenger hunts, road trips or any other such activities carried on outside or inside of the confines of the chapter house; wearing of public apparel which is conspicuous and not normally in good taste; engaging in public stunts and buffoonery; morally degrading or humiliating games and activities; and any other activities which are not consistent with fraternal law, ritual or policy or the regulations and policies of the educational institution." The American College Dictionary goes on to define the action of â€Å"haze† as: â€Å"1. To persecute or harass with meaningless... Free Essays on Hazing Is It Innocent Team Bonding Or The Abuse and Endangerment of An Athlete’s Rights? Current issues have forced NCAA athletics to deal and confront the dangerous acts of team hazing. On January 14th, the University of Vermont men’s hockey season was forced to a sudden halt. With thirteen games left, the team’s 23 remaining players were read a litany of their sins by the state’s attorney general, William Sorrell. He stated the schools handling of the hazing incident(s) was â€Å"conducted loosely while specific evidence was withheld that lead to a slow indecisive investigation,† which resulted in his intervention (Hoover). Hazing is a long practiced and in many cases long accepted rite of initiation in such cliques as military and college fraternities and athletics. It is a relatively new current issue and needs to be seriously addressed. Last August Alfred (NY) University released a study that included two hundred and twenty four participating institutions that submitted names and addresses of 61,258 student athletes. From the responses, they found that 80% of responding college athletes were subjected to alcohol-related hazing and more than 20% to such other activities. Of the participating institutions, the UVM was not one of them (Hoover). Clearly, UVM and other organizations must be more vigilant. Greater and more effective efforts must be made to know what students are doing, and perhaps more importantly, what and how they are thinking. Team or group bonding experiences that attain the desired results without harassing humiliations and dangers must be developed and promoted. Across societies and time, people have initiated new members into groups through ceremonies and rituals designed to foster a feeling of belonging. Yet, sometimes those activities cross the line into hazing. Hazing, defined by Alfred University, is â€Å"any activity expected of someone joining a group that humiliates, degrades, abuses or en... Free Essays on Hazing Hazing In today’s world if a person would want to get on a certain team or join a frat or sorority, that person would have to put up with one big thing, initiation. Or as most call it hazing. There not the only people that put up with hazing though. Hazing is becoming big in high schools, church events, and even professional sports. Since so many people have to put up with the issue of hazing, it has become a very big issue in today’s world. Whether it involves binge drinking, kidnapping, or some type of physical abuse, hazing is becoming an issue that is too common. Since it is becoming so common most people feel that someone needs to step in and stop hazing in all events no matter what it is. Hazing can be defined in many different ways depending on whom you’re talking to. But the actual definition is that it is any activity or situation that has been formed with the intention of causing another person mental or physical pain, embarrassment, or ridicule. Hazing can range from making a person eat ridiculous things, to even have a person take a beating from others. Hazing happens mostly in colleges, but is becoming more common with high schools and other organizations as well. People that want to join a frat or sorority have to deal with this issue on a daily basis, as well as athletes. The idea of hazing is to show how much a certain individual wants to be in a certain club. So since the people at the head of the club can pretty much do what ever they want to a certain individual, they do. They do that because it deals with the universal saying, only the strong survive. Which means the only people that will make it into the club are the ones that do whatever they say. So the heads of the clubs have each person wanting to join do outrageous things because they want to show there power as well as make the person look stupid. But that’s why the heads of these clubs make the statement that it is supposed to g...